초록
<P>Using statistical optimization, we enhanced the activity of phytase by a new <I>Saccharomyces cerevisiae</I> strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2<SUP>2</SUP> full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by <I>S. cerevisiae</I>.</P>