초록
This work aimed at investigating concomitant production of biohydrogen and poly-β-hydroxybutyrate (PHB) by photofermentation (PF) using dark fermentation effluents (DFE). An adapted culture of Rhodobacter sphaeroides AV1b (pH 6.5, 24+/-2<SUP>o</SUP>C) achieved H<SUB>2</SUB> and PHB yields of 256 (+/-2) NmLH<SUB>2</SUB>/g Chemical Oxygen Demand (COD) and 273.8mgPHB/gCOD (32.5+/-3% of the dry cells weight (DCW)), respectively. When a diluted (1:2) DFE medium was applied to the adapted pure and mixed photofermentative culture, the respective H<SUB>2</SUB> yields were 164.0 (+/-12) and 71.3 (+/-6) NmLH<SUB>2</SUB>/gCOD and the PHB yields were 212.1 (+/-105.2) and 50.7 (+/-2.7) mgPHB/gCOD added, corresponding to 24 (+/-0.7) and 6.3 (+/-0) % DCW, respectively. The concomitant H<SUB>2</SUB> and PHB production from the PF process gave a good DFE post treatment achieving up to 80% COD removal from the initial DFE.