Pseudomonas stutzeri lipase: a useful biocatalyst for aminolysis reactions
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Pseudomonas stutzeri lipase: a useful biocatalyst for aminolysis reactions
학술지
Green chemistry : an international journal and green chemistry resource : GC
저자명
van Pelt, S.; Teeuwen, R. L. M.; Janssen, M. H. A.; Sheldon, R. A.; Dunn, P. J.; Howard, R. M.; Kumar, R.; Martí nez, I.; Wong, J. W.
초록
<P>The use of <I>Pseudomonas stutzeri</I> lipase (PSL) as a biocatalyst for aminolysis reactions with bulky substrates has been investigated. PSL compared favorably to Novozym<SUP>®</SUP> 435 (immobilized <I>Candida antarctica</I> lipase B, NOV435) in the aminolysis of various bulky methyl esters and amines. While NOV435 demonstrated a higher rate of aminolysis with methyl 2-phenylpropionic acid as the acyl donor, PSL outperformed NOV435 with secondary amines as the nucleophile. Methanol inhibition and a low affinity for bulky acyl donors were found to be the two main reasons for relatively low rates in the PSL-catalyzed aminolysis reactions. It was demonstrated that the use of molsieve 4A had a significant effect on the aminolysis rate and amide yield, since it enabled the effective removal of the inhibiting methanol from the reaction mixture.</P> <P>Graphic Abstract</P><P>The use of <I>Pseudomonas stutzeri</I> lipase (PSL) as a biocatalyst for aminolysis reactions with bulky substrates has been investigated.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1gc15160f'><BR></P>