BioChem - DOS

Expression of a secretory β-glucosidase from Trichoderma reesei in Pichia pastoris and its characterization

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

Chen, Ping; Fu, Xianyu; Ng, Tzi Bun; Ye, Xiu-Yun

<P>A β-glucosidase gene (bglI) from Trichoderma reesei was cloned into the pPIC9 vector and integrated into the genome of Pichia pastoris GS115. Under the control of the methanol-inducible alcohol oxidase (AOX) promoter and using Saccharomyces cerevisiae secretory signal peptide (α-factor), the recombinant β-glucosidase was expressed and secreted into the culture medium. The maximum recombinant β-glucosidase activity achieved was 60 U/ml, and β-glucosidase expression reached 0.3 mg/ml. The recombinant 76 kDa β-glucosidase was purified 1.8-fold with 26% yield and a specific activity of 197 U/mg. It was optimally active at 70 C and pH 5.0.</P>

2011

Springer-Verlag

0141-5492

1573-6776

33

12

pp.2475-2479

10.1007/s10529-011-0724-3

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