Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

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논문

Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

학술지

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

저자명

Jiang, Yanping; Duarte, Alexandra Vivas; van den Brink, Joost; Wiebenga, Ad; Zou, Gen; Wang, Chengshu; de Vries, Ronald P.; Zhou, Zhihua; Benoit, Isabelle

초록

ObjectivesTo increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.ResultsBy overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnRc/araRc was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.ConclusionsMixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.Electronic supplementary materialThe online version of this article (doi:10.1007/s10529-015-1951-9) contains supplementary material, which is available to authorized users.

발행연도

2016

발행기관

Springer Netherlands

라이선스

cc-by

ISSN

0141-5492

ISSN

1573-6776

권

페이지