초록
<P>Industrial glucose feedstock prepared by enzymatic digestion of starch typically contains significant amounts of disaccharides such as maltose and isomaltose and trisaccharides such as maltotriose and panose. Maltose and maltosaccharides can be utilized in <I>Escherichia coli</I> fermentation using industrial glucose feedstock because there is an intrinsic assimilation pathway for these sugars. However, saccharides that contain α-1,6 bonds, such as isomaltose and panose, are still present after fermentation because there is no metabolic pathway for these sugars. To facilitate more efficient utilization of glucose feedstock, we introduced <I>glvA</I>, which encodes phospho-α-glucosidase, and <I>glvC</I>, which encodes a subunit of the phosphoenolpyruvate-dependent maltose phosphotransferase system (PTS) of <I>Bacillus subtilis</I>, into <I>E. coli.</I> The heterologous expression of <I>glvA</I> and <I>glvC</I> conferred upon the recombinant the ability to assimilate isomaltose and panose. The recombinant <I>E. coli</I> assimilated not only other disaccharides but also trisaccharides, including alcohol forms of these saccharides, such as isomaltitol. To the best of our knowledge, this is the first report to show the involvement of the microbial PTS in the assimilation of trisaccharides. Furthermore, we demonstrated that an <SMALL>L</SMALL>-lysine-producing <I>E. coli</I> harboring <I>glvA</I> and <I>glvC</I> converted isomaltose and panose to <SMALL>L</SMALL>-lysine efficiently. These findings are expected to be beneficial for industrial fermentation.</P>