BioChem - DOS

Biosynthesis of levan by levansucrase from Bacillus methylotrophicus SK 21.002

Carbohydrate polymers

Zhang, Tao; Li, Runjing; Qian, Haobing; Mu, Wanmeng; Miao, Ming; Jiang, Bo

<P><B>Abstract</B></P> <P>A new strain SK 21.002 which could produce intracellular levansucrase was isolated from beet sugar growing soil. It was identified as <I>Bacillus methylotrophicus</I> based on the sequence analysis of the 16S rRNA and the morphological characteristic. The structure of levan biosynthesized from the levansucrase was determined to be connected through &beta;-(2&rarr;6) linkages with fructofuranosyl residues. The molecular weight of the product was between 4 and 5kDa. The optimal conditions to produce levan using the levansucrase were studied. The presence of Mg<SUP>2+</SUP> could increase the yield of levan. The yield of levan reached about 100g/L with the enzyme under the optimum conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel strain SK 21.002 with levansucrase was isolated and identified as <I>B. methylotrophicus</I>. </LI> <LI> The biosynthesized product was identified to be levan. </LI> <LI> The biosynthesis conditions of levan were optimized. </LI> </UL> </P>

2014

Elsevier

0144-8617

101

pp.975-981

10.1016/j.carbpol.2013.10.045

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68181070

Levan; Bacillus methylotrophicus; Levansucrase; Biosynthesis