초록
<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>CO<SUB>2</SUB> is the primary impurity in many fuel gases and may reduce the efficiency of fuel combustion and generate incomplete residues in the product, leading to energy waste and environmental issues after emission. Currently although plants/microalgae‐mediated approaches have been widely used for CO<SUB>2</SUB> separation/digestion, they usually emphasize CO<SUB>2</SUB> removal efficacy for the purpose of environment protection, but give less consideration to subsequent CO<SUB>2</SUB> manipulation for cell culture that restricts their effectiveness thereafter. To address the above issues, a perfluorinated photobioreactor system (PPBRS) enabling CO<SUB>2</SUB> separation and controlled microalgae cultivation was developed in this study.</P><P><B>RESULTS</B></P><P>Results showed that the PPBRS provided >85% CO<SUB>2</SUB> separation efficiency from 60%‐N<SUB>2</SUB>/40%‐CO<SUB>2</SUB> within 5 days by using perfluorocarbon as the CO<SUB>2</SUB> absorbent. <I>Nannochloropsis oculata</I> cultured with 20 mL min<SUP>‐1</SUP> perfluorocarbon containing 2% CO<SUB>2</SUB> isolated from the gas mixture exhibited 2‐fold higher cell concentration and yielded 1.8‐, 2.5‐, and 2‐fold (<I>P</I> < 0.05 for all) increased productions of biomass, lipid, and eicosapentaenoic acid, respectively, compared with the group with air aeration without PFC for 5 days.</P><P><B>CONCLUSION</B></P><P>Perfluorocarbon is recyclable and all procedures including CO<SUB>2</SUB> extraction, collection of purified gas, and delivery of isolated CO<SUB>2</SUB> to cell culture are a continuous process without need for additional handling. The PPBRS thus provides an efficacious, easy‐to‐use, and cost‐effective means for simultaneous CO<SUB>2</SUB> separation and enhanced microalgae production that can be applied for use in industry. © 2017 Society of Chemical Industry</P>