초록
The potential of fungal pretreatment prior to acetic acid pretreatment using Phanerochaete chrysosporium for enzymatic hydrolysis and algal oil production from Glycyrrhiza uralensis residue (GUR) was investigated. After co-pretreatment with P. chrysosporium and acetic acid, the loss of lignin and holocellulose was 47.4% and less than 28%, respectively. Subsequently, the acid insoluble residues were further hydrolyzed with cellulase into fermentable sugars, and then used for heterotrophic cultivation of Chlorella protothecoides for bio-oils production. The concentration of reducing sugars in enzymatic hydrolyzate from co-treated GUR was 1.62 times as that of solely acid pretreated GUR. The maximum yield of algal biomass and oil from co-pretreatment (4.46 and 1.91g/L) were both higher than those from sole acid pretreatment. Thus, this study demonstrated the feasibility of using reducing sugars obtained from enzymatic hydrolysis of GUR co-treated with P. chrysosporium and acetic acid as substrate for oil production.