<P><B>Abstract</B></P> <P>This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (<I>BGL</I>, <I>XYNII</I>, <I>EGII</I>, <I>CBHI</I> and <I>CBHII</I>) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93g/l ethanol whiles that for LUKP produced 0.71g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cocktail δ-integration was used for the introduction of cellulase genes. </LI> <LI> Various consolidated bioprocess (CBP) yeast strains resulted. </LI> <LI> Ethanol production by CBP strains from different biomasses varied. </LI> <LI> A CBP yeast strain produced 91.2% ethanol from ionic liquid-pretreated bagasse. </LI> <LI> Copy numbers of integrated genes varied from transformant to transformant. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>