BioChem - DOS

Reconstruction of the Biosynthetic Pathway of Santalols under Control of the GAL Regulatory System in Yeast

ACS Synthetic biology

Zha, Wenlong; An, Tianyue; Li, Ting; Zhu, Jianxun; Gao, Ke; Sun, Zhenjiao; Xu, Wendong; Lin, Pengcheng; Zi, Jiachen

<P>Sandalwood oil has been widely used in perfumery industries and aromatherapy. Santalols are its major components. Herein, we attempted to construct santalol-producing yeasts. To alter flux from predominant triterpenoid/steroid biosynthesis to sesquiterpenoid production, expression of <I>ERG9</I> (encoding yeast squalene synthase) was depressed by replacing its innate promotor with <I>P</I><SUB><I>HXT1</I></SUB> and fermenting the resulting strains in galactose-rich media. And the genes related to santalol biosynthesis were overexpressed under control of <I>GAL</I> promotors, which linked santalol biosynthesis to GAL regulatory system. GAL4 (a transcriptional activator of <I>GAL</I> promotors) and PGM2 (a yeast phosphoglucomutase) were overexpressed to overall promote this artificial santalol biosynthetic pathway and enhance galactose uptake. 1.3 g/L santalols and 1.2 g/L <I>Z</I>-&alpha;-santalol were achieved in the strain WL17 expressing SaSS (&alpha;-santalene synthase from <I>Santalum album</I>) and WL19 expressing SanSyn (&alpha;-santalene synthase from <I>Clausena lansium</I>) by fed-batch fermentation, respectively. This study constructed the microbial santalol-producing platform for the first time.</P><BR>[FIG OMISSION]</BR>

2020

American Chemical Society

2161-5063

9

2

pp.449-456

10.1021/acssynbio.9b00479.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98897486

https://doi.org/10.1021/acssynbio.9b00479.s001

sandalwood oil; sesquiterpenoid; santalol; GAL regulatory system; PGM2; yeast