BioChem - DOS

Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cheng, J.; Liu, M.; Song, W.; Ding, L.; Liu, J.; Zhang, L.; Cen, K.

Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of <SUP>60</SUP>Co &gamma;-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4mLH<SUB>2</SUB>/(gDWh). The hereditary stability of the E. aerogenes ZJU1 mutant was certified after over ten generations of cultivation. The hydrogen yield of 301mLH<SUB>2</SUB>/gglucose with the mutant was higher by 81.8% than that of 166mL/gglucose with the wild strain. The peak hydrogen production rate of 27.2mL/(L.h) with the mutant was higher by 40.9% compared with that of 19.3mL/(L.h) with the wild strain. The mutant produced more acetate and butyrate but less ethanol compared with the wild strain during hydrogen fermentation.

2017

Elsevier Applied Science

0960-8524

227

pp.50-55

10.1016/j.biortech.2016.12.033

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77178962

Hydrogen; Enterobacter aerogenes; Nuclear irradiation; Bacteria mutant