초록
Enhancing substrate accessibility is one of the prerequisites for efficient pretreatment and bioconversion of lignocellulosic biomass, for which reducing particle size and increasing porosity have been implemented. Biomass porosity, characterized as the water retention capacity (WRC), increases significantly with the use of a culture filtrate of an H<SUB>2</SUB>-producing microbial consortium, causing native lignocellulosic fibers to hydrate and swell extensively. This study describes the effects of hydration treatment and particle size on the biodegradability of native wheat straw fibers with regard to direct H<SUB>2</SUB> production by a microbial consortium. A culture filtrate and tap water were used as hydration media, and particle sizes of 0.212 and 3.35 mm were tested. As a result, biodegradability, measured as H<SUB>2</SUB> production, doubled when the lignocellulosic substrate was hydrated with the culture filtrate. Also, hydration had a significant impact on H<SUB>2</SUB> yield, increasing it from 6.8 to 86.5 mL H<SUB>2</SUB>/g total consumed sugars when the 0.212 mm fibers were first hydrated. H<SUB>2</SUB> production was higher with coarser particles (3.35 > 0.212 mm), but hydrogen yield improved with finer particles (0.212 > 3.35 mm). Based on our results, particle size and hydration improve the biodegradability of native wheat straw for H<SUB>2</SUB> production, although hydration with a culture filtrate increased the biomass porosity (measured as WRC), which influenced the biodegradability more than a reduction in particle size.