BioChem - DOS

Efficient succinic acid production using a biochar-treated textile waste hydrolysate in an in situ fibrous bed bioreactor

Biochemical engineering journal

Li, Xiaotong; Zhang, Ming; Luo, Jiewen; Zhang, Shicheng; Yang, Xiao; Igalavithana, Avanthi Deshani; Ok, Yong Sik; Tsang, Daniel C.W.; Lin, Carol Sze Ki

<P><B>Abstract</B></P> <P>Textile waste contains biodegradable fraction which can be used as an alternative feedstock for succinic acid production. The feasibility of bioconversion of biochar-treated textile waste hydrolysate into succinic acid was evaluated. A substrate loading of 9% was applied to hydrolyze mixed textile waste and the resultant glucose-rich hydrolysate with dyestuff was collected after enzymatic hydrolysis. Biochar from different pyrolysis temperatures (400&ndash;700 &deg;C) and different dosages of the selected biochar (1&ndash;5%) were applied to remove colorants. The results showed that biochar had a good performance at a dosage of 2 w/w % on removal of colorant inhibitors. There was no negative effect observed during the subsequent fermentation. After optimization of fermentation media in shake flasks, the resultant succinic acid titer reached 19.6 g/L with a SA yield of 0.76 g/g, supplemented with 30 g/L tryptone. The <I>in situ</I> fibrous bed bioreactor (<I>is</I>FBB) can further improve SA titer, up to 28.8 g/L corresponding to a yield of 0.61 g/g without pH control. Furthermore, a shorter lag phase during succinic acid fermentation in <I>is</I>FBB was observed. It can be concluded that a novel textile waste-based biorefinery approach for succinic acid production aided by biochar sorption was successfully developed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Utilization of biochar for removal of dyestuff from textile waste hydrolysate. </LI> <LI> Novel textile waste-based biorefinery strategy for succinic acid production. </LI> <LI> Optimization of fermentation medium for SA production by <I>Yarrowia lipolytica</I>. </LI> <LI> Enhanced succinic acid production using an <I>in-situ</I> fibrous bed bioreactor. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2019

Elsevier

1369-703x

149

pp.107249

10.1016/j.bej.2019.107249

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97555286

Biochar; In situ fibrous bed bioreactor; Succinic acid; Textile waste; Yarrowia lipolytica