Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass

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- 바이오정밀화학
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논문

Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Li, Y.; Qi, B.; Wan, Y.

초록

Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC<SUB>50</SUB> was estimated to be 30g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase.

발행연도

2014

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

167

페이지

pp.324-330

DOI

10.1016/j.biortech.2014.06.035