BioChem - DOS

Optimization of Salts Supplementation on Xylitol Production by Debaryomyces hansenii Using a Synthetic Medium or Corncob Hemicellulosic Hydrolyzates and Further Scaled Up

Industrial & engineering chemistry research

Bustos Vá zquez, Guadalupe; Pé rez-Rodrí guez, Noelia; Salgado, José Manuel; Oliveira, Ricardo Pinheiro de Souza; Domí nguez, José Manuel

<P>The synergic effect of eight salts on <SMALL>d</SMALL>-xylose to xylitol bioconversion by <I>Debaryomyces hansenii</I> NRRL Y-7426 was optimized through a central composite design (CCD) in shaker flasks. Optima conditions were then assayed using detoxified corncob hemicellulosic hydrolyzates as the carbon source, increasing the fermentative parameters to <I>Q</I><SUB>P</SUB> = 0.118 g/L&middot;h and <I>Y</I><SUB>P/S</SUB> = 0.45 g/g from <I>Q</I><SUB>P</SUB> = 0.101 g/L&middot;h and <I>Y</I><SUB>P/S</SUB> = 0.39 g/g (without supplementation). Finally, the process was successfully scaled up to a 50 L Braun Biostat D50 fermenter, since the time of fermentation was reduced from 103 h to 42.8 h and the fermentation parameters increased to <I>Q</I><SUB>P</SUB> = 0.395 g/L&middot;h and <I>Y</I><SUB>P/S</SUB> = 0.59 g/g using a synthetic medium and <I>Q</I><SUB>P</SUB> = 0.236 g/L&middot;h and <I>Y</I><SUB>P/S</SUB> = 0.39 g/g with detoxified hydrolyzates. All the results confirmed the positive influence of supplementing synthetic culture media or detoxified corncob hydrolyzates with salts for the development of <I>D. hansenii</I>.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/iecred/2017/iecred.2017.56.issue-23/acs.iecr.7b01120/production/images/medium/ie-2017-011207_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ie7b01120'>ACS Electronic Supporting Info</A></P>

2017

American Chemical Society

0888-5885

1520-5045

56

23

pp.6579-6589

10.1021/acs.iecr.7b01120.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78034760

https://doi.org/10.1021/acs.iecr.7b01120.s001