Enhancing of sugar cane bagasse hydrolysis by Annulohypoxylon stygium glycohydrolases

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오정밀화학
  1. 기타

논문

Enhancing of sugar cane bagasse hydrolysis by Annulohypoxylon stygium glycohydrolases

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Robl, D.; dos Santos Costa, P.; Buchli, F.; da Silva Lima, D.J.; da Silva Delabona, P.; Squina, F.M.; Pimentel, I.C.; Padilla, G.; da Cruz Pradella, J.G.

초록

The aim of this study was to develop a bioprocess for the production of β-glucosidase and pectinase from the fungus Annulohypoxylon stygium DR47. Media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26U/mL of pectinase at pH 4.0 and 10.13U/mL of β-glucosidase at pH 5.0. In addition, the enzymes extracts were able to replace partially Celluclast 1.5L in sugar cane bagasse hydrolysis. Proteomic analysis from A. stygium cultures revealed accessory enzymes, mainly belong to the families GH3 and GH54, that would support enhancement of commercial cocktail saccharification yields. This is the first report describing bioreactor optimization for enzyme production from A. stygium with a view for more efficient degradation of sugar cane bagasse.

발행연도

2015

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

177

페이지

pp.247-254

DOI

10.1016/j.biortech.2014.11.082