초록
<P><B>Abstract</B></P><P><B>Aims</B></P><P>To characterize the hemicellulolytic and cellulolytic enzymes from novel fungi, and evaluate the potential of novel enzyme system in releasing ferulic acid (FA) from biomass resource.</P><P><B>Methods and Results</B></P><P>A hemicellulolytic and cellulolytic enzyme‐producing fungus 4‐14 was isolated from soil by Congo red staining method, and identified as <I>Eupenicillium parvum</I> based on the morphologic and molecular phylogenetic analysis. The optimum temperature of fungal growth was 37°C. Hemicellulolytic and cellulolytic enzymes were produced by this fungus in solid‐state fermentation (SSF), and their maximum activities were 554, 385, 218, 2·62 and 5·25 U g<SUP>−1</SUP> for CMCase, xylanase, <I>β</I>‐glucosidase, FPase and FAE respectively. These enzymes displayed the best catalytic ability at low pH values (pH 4·5–5·0). The optimum temperatures were 70°C, 70°C, 75°C and 55°C for CMCase, <I>β</I>‐glucosidase, xylanase and FAE respectively. CMCase, xylanase and FAE were stable at different pHs or high temperature (60°C). Enzymatic hydrolysis experiment indicated that the maximum (76·8 ± 4)% of total alkali‐extractable FA was released from de‐starched wheat bran by the fungal enzyme system.</P><P><B>Conclusions</B></P><P>High activities of thermotolerant CMCase, <I>β</I>‐glucosidase, xylanase and FAE were produced by the newly isolated fungus <I>E. parvum</I> 4‐14 in SSF. The fungal enzyme system displayed high efficiency at releasing FA from wheat bran.</P><P><B>Significance and Impact of the Study</B></P><P>This study provides a new fungal strain for researches of novel hemicellulolytic and cellulolytic enzymes and will improve the bioconversion and utilization of agricultural by‐products.</P>