BioChem - DOS

The hydrolytic efficiency and synergistic action of recombinant xylan-degrading enzymes on xylan isolated from sugarcane bagasse

Carbohydrate polymers

Li, Hailong; Wu, Hongli; Xiong, Lian; Chen, Xuefang; Wang, Can; Qi, Gaoxiang; Huang, Chao; Guo, Haijun; Luo, Mutan; Liu, Jian; Long, Minnan; Chen, Xinde

<P><B>Abstract</B></P> <P>Understanding the interaction mechanisms between xylan and xylan-degrading enzymes is beneficial to the efficient hydrolysis of xylan. Xylan from sugarcane bagasse (SB) was extracted and characterized. The effects of heat treatment and removal of side chains of SB xylan on the hydrolytic efficiency and synergistic action of endo-&beta;-1,4-xylanases (HoXyn11A and AnXyn10C), &beta;-xylosidases (AnXln3D), and &alpha;-<SMALL>L</SMALL>-arabinofuranosidases (AnAxh62A) were investigated. Results indicated that heat treatment of xylan can improve the hydrolytic efficiency of xylan-degrading enzymes, and it is essential for the efficient hydrolysis of xylan by HoXyn11A. The removal of arabinofuranosyl side chains of xylan by AnAxh62A before enzymatic hydrolysis reduced the hydrolytic efficiency of HoXyn11A and AnXyn10C on xylan. AnXyn10C was more efficient than HoXyn11A in hydrolysis of xylan, whereas HoXyn11A showed better synergistic action than AnXyn10C with AnAxh62A and AnXln3D in the hydrolysis of xylan. This study provides new insights on the enzymatic hydrolysis of SB into monosaccharides and xylo-oligosaccharides.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Heat treatment of xylan improved the hydrolytic efficiency of xylan-degrading enzymes. </LI> <LI> The removal of &alpha;-L-Ara<I>f</I> side chains of xylan reduced the hydrolytic efficiency of EXs. </LI> <LI> The synergistic action of recombinant enzymes on xylan extracted from SB was studied. </LI> </UL> </P>

2017

Elsevier

0144-8617

175

pp.199-206

10.1016/j.carbpol.2017.07.075

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78541360

Hydrolytic efficiency; Synergistic action; Sugarcane bagasse; Xylan; Xylan-degrading enzymes