초록
1,3-Propanediol (1,3-PD) is widely used in polymer industry in production of polyethers, polyesters and polyurethanes. In this article, a study on 1,3-PD production and tolerance of Halanaerobium saccharolyticum subsp. saccharolyticum is presented. 1,3-PD production was optimized for temperature, vitamin B<SUB>12</SUB> and acetate concentration. The highest 1,3-PD concentrations and yields (0.6mol/mol glycerol) were obtained at vitamin B<SUB>12</SUB> concentration 64μg/l and an inverse correlation between 1,3-PD and hydrogen production was observed with varying vitamin B<SUB>12</SUB> concentrations. In the studied temperature range and initial acetate concentrations up to 10g/l, no significant variations were observed in 1,3-PD production. High initial acetate (29-58g/l) was observed to cause slight decrease in 1,3-PD concentrations produced but no effects on 1,3-PD yields (mol/mol glycerol). Initial 1,3-PD concentrations inhibited the growth of H. saccharolyticum subsp. saccharolyticum. When initial 1,3-PD concentration was raised from 1g/l to 57g/l, a decrease of 12% to 75%, respectively, in the highest optical density was observed.