BioChem - DOS

Analysis of neofructooligosaccharides production mediated by the extracellular β-fructofuranosidase from Xanthophyllomyces dendrorhous

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Linde, Dolores; Rodrí guez-Colinas, Barbara; Esté vez, Marta; Poveda, Ana; Plou, Francisco J.; Ferná ndez Lobato, Marí a

<P><B>Highlights</B></P><P>&#x025BA; Extracellular fructofuranosidase from <I>Xanthophyllomyces</I> produces two FOS series. &#x025BA; Different glycoforms of the fructofuranosidase Xd-INV as neo-FOS producers. &#x025BA; Neo-FOS production improved 4-fold altering temperature and substrate concentration. &#x025BA; The largest neo-FOS production reported to date for any single microbial enzyme.</P> <P><B>Abstract</B></P><P>The extracellular &beta;-fructofuranosidase Xd-INV from the yeast <I>Xanthophyllomyces dendrorhous</I> mainly synthesizes the neo-fructooligosaccharides (neo-FOS) neokestose and neonystose. This enzyme is a glycoprotein with a content of 59&ndash;67% N-linked carbohydrates and an estimated molecular mass of 160&ndash;200kDa. The extent level of glycosylation affects the thermal behaviour of the enzyme but not its hydrolase and transferase activities, which are optimal at 60&ndash;70&deg;C. The neo-FOS yield of this enzyme was increased from 40 to 168g/L when the sucrose concentration increased from 420 to 600g/L and when the reaction was carried out at 60&deg;C. The neo-FOS levels obtained (168g/L) in this work are the largest reported for any microbial &beta;-fructofuranosidase.</P>

2012

0960-8524

109

pp.123-130

10.1016/j.biortech.2012.01.023

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58595347

Extracellular &beta; -fructofuranosidase; Xanthophyllomyces dendrorhous; Neofructooligosaccharides; Prebiotics;