BioChem - DOS

Simple and Practical Multigram Synthesis of D-Xylonate Using a Recombinant Xylose Dehydrogenase

ACS omega

Sá nchez-Moreno, Israel; Benito-Arenas, Raú l; Montero-Calle, Pilar; Hermida, Carmen; Garcí a-Junceda, Eduardo; Ferná ndez-Mayoralas, Alfonso

<P>An efficient multienzyme system for the preparative synthesis of <SMALL>D</SMALL>-xylonate, a chemical with versatile industrial applications, is described. The multienzyme system is based on <SMALL>D</SMALL>-xylose oxidation catalyzed by the xylose dehydrogenase from <I>Calulobacter crescentus</I> and the use of catalytic amounts of NAD<SUP>+</SUP>. The cofactor is regenerated in situ by coupling the reduction of acetaldehyde into ethanol catalyzed by alcohol dehydrogenase from <I>Clostridium kluyveri</I>. Excellent conversions (>95%) were obtained in a process that allows easy product isolation by simple evaporation of the volatile buffer and byproducts.</P><BR>[FIG OMISSION]</BR>

2019

American Chemical Society

acs-specific: authorchoice/editors choice usage agreement

2470-1343

4

6

pp.10593-10598

10.1021/acsomega.9b01090

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97802135

https://pubs.acs.org/doi/pdf/10.1021/acsomega.9b01090