BioChem - DOS

Optimization of Lipase Biosynthesis from Rhizopus oryzae for Biodiesel Production Using Multiple Oils

Chemical engineering & technology

Mukhtar, Hamid; Khursheed, Samreen; Ikram‐ ul‐ Haq,; Mumtaz, Muhammad Waseem; Rashid, Umer; Al‐ Resayes, Saud Ibrahim

<P><B>Abstract</B></P><P>Lipase synthesis was optimized using different substrates through solid&#8208;state fermentation followed by application of the produced lipase for biodiesel synthesis from multiple oils. <I>Rhizopus oryzae</I> RO&#8208;1 showed maximum lipase production and was selected for further use. The production of lipase by <I>R. oryzae</I> RO&#8208;1 could be improved when the substrate was supplemented with olive oil and the growth conditions were optimized. After partial purification, the enzyme was immobilized on four different supports. For the production of biodiesel, Amberlite&#8208;immobilized lipase was employed for transesterification of olive, canola, sunflower, soybean, and algal oil from <I>Cladophora</I> sp. The biodiesels produced were characterized by recording their Fourier transform infrared spectra. Biodiesel generated from algal oil contains high levels of polyunsaturated fatty acids, making it highly suitable as winter&#8208;grade biodiesel.</P>

2016

WILEY&#x2010;VCH Verlag

0930-7516

1521-4125

39

9

pp.1707-1715

10.1002/ceat.201500584

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76223134

Algal oil; Biodiesel production; Lipase biosynthesis; Solid&#x2010; state fermentation; Transesterification;