BioChem - DOS

Investigation on hydrogen production from paper sludge without inoculation and its enhancement by Clostridium thermocellum

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

An, Qian; Wang, Ji-Lian; Wang, Yu-Tao; Lin, Zhang-Lin; Zhu, Ming-Jun

<P><B>Abstract</B></P> <P>The feasibility and performance of hydrogen production from paper sludge without inoculation was investigated under thermophilic conditions. The maximum hydrogen production reached 64.32 mM with 7.4% PS. The dynamic changes in bacterial community structures during hydrogen production were investigated by analyzing 16S rDNA gene sequences using high throughput sequencing technology. The results showed that microbial community was dominated by order <I>Clostridiales</I> and <I>Thermoanaerobacterales</I>. Genus <I>Thermoanaerobacterium</I> and <I>Ruminiclostridium</I> played a leading role in the fermentation process, which was responsible for the hydrolysis of PS and hydrogen production. Effect of inoculation with <I>Clostridium thermocellum</I> on hydrogen production from PS was also studied. The results showed that <I>C. thermocellum</I> supplement significantly increased hydrogen yield and holocellulose degradation rate by 96.80% and 32.95%, respectively. In addition, inoculation of <I>C. thermocellum</I> enhanced VFA generation and shortened the lag phase of hydrogen production. The present study lays the foundation on the valorization of waste lignocellulose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Ruminiclostridium</I> and <I>Thermoanaerobacterium</I> dominated in non-inoculation process. </LI> <LI> <I>C. thermocellum</I> inoculation enhanced H<SUB>2</SUB> yield dramatically. </LI> <LI> The substrate loading was optimized in <I>C. thermocellum</I> inoculation process. </LI> </UL> </P>

2018

Elsevier

0960-8524

263

pp.120-127

10.1016/j.biortech.2018.04.105

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART85386091

Paper sludge; Hydrogen production; Bacterial community; Clostridium thermocellum; Enhancement