Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and ethanol production

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논문

Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and ethanol production

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

de Cassia Pereira, J.; Travaini, R.; Paganini Marques, N.; Bolado-Rodriguez, S.; Bocchini Martins, D.A.

초록

The saccharification of ozonated sugarcane bagasse (SCB) by enzymes from Myceliophthora thermophila JCP 1-4 was studied. Fungal enzymes provided slightly higher sugar release than commercial enzymes, working at 50<SUP>o</SUP>C. Sugar release increased with temperature increase. Kinetic studies showed remarkable glucose release (4.99g/L, 3%w/w dry matter) at 60<SUP>o</SUP>C, 8h of hydrolysis, using an enzyme load of 10FPU (filter paper unit). FPase and β-glucosidase activities increased during saccharification (284% and 270%, respectively). No further significant improvement on glucose release was observed increasing the enzyme load above 7.5FPUperg of cellulose. Higher dry matter contents increased sugars release, but not yields. The fermentation of hydrolysates by Saccharomyces cerevisiae provided glucose-to-ethanol conversions around to 63%.

발행연도

2016

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

204

페이지

pp.122-129

DOI

10.1016/j.biortech.2015.12.064