초록
<P><B>Abstract</B></P><P>Two bacterial strains able to produce polyhydroxyalkanoates (PHAs) from a wide variety of pure carbon sources (dextrose, xylose, sucrose, lactose and glycerol) were isolated from forest soils and identified as <I>Achromobacter mucicolens</I> and <I>Stenotrophomonas rhizophila. Achromobacter mucicolens</I> also produced poly(3‐hydroxybutyrate) (PHB) from different wastes (cheese whey, molasses, agave bagasse hydrolysate, nejayote and mango waste pulp). <I>Stenotrophomonas rhizophila,</I> produced the copolymer poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHB<I>‐co‐</I>HV) from glycerol (7·7 mol% of HV), and from sucrose with addition of propionic or valeric acid (4·5 and 25 mol% of HV, respectively). The copolymers presented a lower melting point (145, 156 and 127°C) and crystallinity (23, 26 and 16%) than PHB. The maximum biopolymer accumulation (PHB) for each strain growing in pure carbon source was as follows: 31·3 g per 100 g dry cell weight (DCW) for <I>A. mucicolens</I> from xylose; and 13·7 g per 100 g DCW for <I>S. rhizophila</I> from sucrose. Regarding the waste carbon sources, the highest PHB accumulation was obtained from agave bagasse hydrolysate (20·4 g per 100 g DCW) by <I>A. mucicolens</I>. The molecular weights of the biopolymers obtained ranged from 200 to 741 kDa.</P><P><B>Significance and Impact of the Study</B></P><P>The economic cost of the carbon source for the culture of polyhydroxyalkanoates (PHAs)‐producing microorganisms is one of the main process limitations. Therefore, it is vital to find versatile microorganisms able to grow and to accumulate homo and copolymers of PHAs from low‐cost substrates. In this research, we report two bacterial strains that produce poly(3‐hydroxybutyrate), poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) or both from at least five pure and five waste carbon sources. These results, by such bacterial strains have not been reported, especially the production of copolymer from glycerol without addition of precursors by <I>Stenotrophomonas rhizophila</I> and the production of PHB from xylose and agave bagasse hydrolysate by <I>Achromobacter mucicolens</I>.</P>