Supplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity

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논문

Supplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Singhvi, Mamata; Jadhav, Akanksha; Gokhale, Digambar

초록

Abstract The production of D-lactic acid by Lactobacillus lactis RM2-24 was investigated using modified media to increase the efficiency of the fermentation process. The results indicated that the addition of 5g/l peptone and 1g/l (NH4)2HPO4 enhanced D-lactic acid production by 32%, as compared to that obtained from non supplemented media, with a productivity of 3.0g/l/h. Lactate dehydrogenase (LDH) expression profile in these different media was studied which resulted in appearance of additional LDH isoform produced by cells when they were grown in HSYE supplemented with (NH4)2HPO4. The additional LDH appears to be L-LDH contributing to production of L-lactic acid in the fermented broth. This is totally new information in the lactic acid fermentation and could be very useful to industries engaged in D-lactic acid production. Highlights <UL> <LI> Addition of (NH4)2HPO4 and peptone in media enhanced D-lactic acid production. </LI> <LI> (NH4)2HPO4 induces L-lactate dehydrogenase in D-lactic acid producing strain. </LI> <LI> This is the novel finding and new microbiological information in D-lactic acid fermentation. </LI> <LI> These findings are very useful to industries engaged in lactic acid production. </LI> </UL>

발행연도

2013

발행기관

Elsevier

ISSN

0960-8524

권

146

페이지

pp.736-739

DOI

10.1016/j.biortech.2013.07.057