BioChem - DOS

Integrated chemical and multi-scale structural analyses for the processes of acid pretreatment and enzymatic hydrolysis of corn stover

Carbohydrate polymers

Chen, L.; Li, J.; Lu, M.; Guo, X.; Zhang, H.; Han, L.

Corn stover was pretreated with acid under moderate conditions (1.5%, w/w, 121<SUP>o</SUP>C, 60min), and kinetic enzymolysis experiments were performed on the pretreated substrate using a mixture of Celluclast 1.5L (20FPU/g dry substrate) and Novozyme 188 (40CBU/g dry substrate). Integrated chemical and multi-scale structural methods were then used to characterize both processes. Chemical analysis showed that acid pretreatment removed considerable hemicellulose (from 19.7% in native substrate to 9.28% in acid-pretreated substrate) and achieved a reasonably high conversion efficiency (58.63% of glucose yield) in the subsequent enzymatic hydrolysis. Multi-scale structural analysis indicated that acid pretreatment caused structural changes via cleaving acetyl linkages, solubilizing hemicellulose, relocating cell wall surfaces and enlarging substrate porosity (pore volume increased from 0.0067cm<SUP>3</SUP>/g in native substrate to 0.019cm<SUP>3</SUP>/g in acid-pretreated substrate), thereby improving the polysaccharide digestibility.

2016

Applied Science Publishers ; Elsevier Science Ltd

0144-8617

141

pp.1-9

10.1016/j.carbpol.2015.12.079

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74934374

Acid pretreatment; Chemical analysis; Corn stover; Enzymatic hydrolysis; Structural analysis