BioChem - DOS

Farnesol stimulates laccase production in Trametes versicolor

Engineering in life sciences

Hu, Jianhua; Wang, Feng; Ma, Anzhou; Zhuang, Guoqiang; Liu, Ying; Lu, Jingsong; Guo, Chen; Liu, Chunzhao

<P>Farnesol, a quorum&#8208;sensing molecule, was used successfully to improve laccase production in submerged cultures of <I>Trametes versicolor</I>. At the optimal farnesol concentration of 60 &mu;M added at the beginning of the culture, the extracellular laccase activity reached 629.3 U L<SUP>&minus;1</SUP> after 6 days of cultivation, which represented a 1.92&#8208;fold increase relative to the control without farnesol treatment. The addition of farnesol resulted in an increase in the accumulation of H<SUB>2</SUB>O<SUB>2</SUB> and an increased expression of the laccase (<I>lac</I>) gene and the <I>RhoA</I> gene. The <I>RhoA</I> gene correlated with hyperbranched mycelia, which facilitated the secretion of the intracellular laccase. This study provides a basis for understanding the induction mechanism of farnesol for enhancing laccase production.</P>

2016

1618-0240

1618-2863

16

4

pp.364-370

10.1002/elsc.201500082

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75450820

Farnesol; Hyperbranch; Laccase; Quorum sensing; Trametes versicolor