BioChem - DOS

A standardized biohydrogen potential protocol: An international round robin test approach

International journal of hydrogen energy

Carrillo-Reyes, Juliá n; Tapia-Rodrí guez, Aí da; Buitró n, Germá n; Moreno-Andrade, Ivá n; Palomo-Briones, Rodolfo; Razo-Flores, Elí as; Aguilar Juá rez, Oscar; Arreola-Vargas, Jorge; Bernet, Nicolas; Maluf Braga, Adriana Ferreira; Braga, Lucia; Castelló , Elena; Chatellard, Lucile; Etchebehere, Claudia; Fuentes, Laura; Leó n-Becerril, Elizabeth; Mé ndez-Acosta, Hugo Oscar; Ruiz-Filippi, Gonzalo; Tapia-Venegas, Estela; Trably, Eric; Wenzel, Jorge; Zaiat, Marcelo

<P><B>Abstract</B></P> <P>Hydrogen production by dark fermentation is an emerging technology of increasing interest due to its renewable feature. Recent scientific advances have well investigated the operational conditions to produce hydrogen through the valorization of several wastes or wastewaters. However, the development of standardized protocols to accurately assess the biohydrogen potential (BHP) is of crucial importance. This work is the first interlaboratory and international effort to validate a protocol estimating hydrogen potential using batch tests, using glucose as individual model substrate. The repeatability of the hydrogen potential (HP) increased with variations of the proposed protocol: reducing substrate concentration, increasing the buffer capacity, and using an automatic device. The interlaboratory variation of the HP was reduced from 32 to 12%, demonstrating the reproducibility and robustness of the proposed protocol. Recommendations to run BHP tests were formulated in terms of i) repeatability and reproducibility of results, ii) criteria for results validation and acceptance, iii) workload of the proposed protocols.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The standardized protocol for bioH<SUB>2</SUB> potential was internationally validated. </LI> <LI> Substrate content and buffer capacity affect the repeatability and reproducibility. </LI> <LI> H<SUB>2</SUB> potential fits the acceptance criteria of &ge;14% of interlaboratory variation. </LI> <LI> Either the manual or automatic protocol fits the acceptance criteria. </LI> </UL> </P>

2019

Elsevier

0360-3199

1879-3487

44

48

pp.26237-26247

10.1016/j.ijhydene.2019.08.124

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97998343

https://hal.inrae.fr/hal-02623601/document

Biohydrogen; Batch protocol; Dark fermentation; Glucose; Heat-treated inoculum