초록
<P><B>Abstract</B></P> <P>Three strategies were presented to achieve high solids loading while maximizing carbohydrate conversion, which are fed-batch, splitting/thickening, and clarifier processes. Enzymatic hydrolysis was performed at water insoluble solids (WIS) of 15% using washed dilute-acid pretreated corn stover. The carbohydrate concentration increased from 31.8 to 99.3g/L when the insoluble solids content increased from 5% to 15% WIS, while the final carbohydrate conversion was decreased from 78.4% to 73.2%. For the fed-batch process, a carbohydrate conversion efficiency of 76.8% was achieved when solid was split into 60:20:20 ratio, with all enzymes added first. For the splitting/thickening process, a carbohydrate conversion of 76.5% was realized when the filtrate was recycled to simulate a steady-state process. Lastly, the clarifier process was evaluated and the highest carbohydrate conversion of 81.4% was achieved. All of these results suggests the possibility of enzymatic hydrolysis at high solids to make the overall conversion cost-competitive.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three strategies for high-solids enzymatic hydrolysis were evaluated. </LI> <LI> Fed-batch can effectively reduce viscosity and increase initial hydrolysis rate. </LI> <LI> Splitting/thickening can effectively mix enzymes into pretreated substrate. </LI> <LI> Clarifier can reduce initial viscosity at similar final sugar concentration. </LI> </UL> </P>