Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp. to Improve Polyunsaturated Fatty Acid Production

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- 바이오정밀화학
  1. 용매
  2. 화학제품
- 화장품용 기능성소재
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  2. 건강보조식품

논문

Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp. to Improve Polyunsaturated Fatty Acid Production

학술지

Journal of agricultural and food chemistry

저자명

Li, Zhipeng; Meng, Tong; Ling, Xueping; Li, Jun; Zheng, Chuqiang; Shi, Yanyan; Chen, Zhen; Li, Zhenqi; Li, Qingbiao; Lu, Yinghua; He, Ning

초록

Polyunsaturated fatty acids (PUFAs) have been widely applied in the food and medical industry. In this study, malonyl-CoA: ACP transacylase (MAT) was overexpressed through homologous recombination to improve PUFA production in Schizochytrium. The results showed that the lipid and PUFA concentration were increased by 10.1 and 24.5% with MAT overexpression, respectively. Metabolomics analysis revealed that the intracellular tricarboxylic acid cycle was weakened and glucose absorption was accelerated in the engineered strain. In the mevalonate pathway, intracellular carotene content was decreased, and the carbon flux was then redirected toward PUFA synthesis. Furthermore, a glucose fed-batch fermentation was finally performed with the engineered Schizochytrium. The total lipid yield was further increased to 110.5 g/L, 39.6% higher than the wild strain. Docosahexaenoic acid and eicosapentaenoic acid yield were enhanced to 47.39 g/L and 1.65 g/L with an increase of 81.5 and 172.5%, respectively. This study provided an effective metabolic engineering strategy for industrial PUFA production. [FIG OMISSION]

발행연도

2018

발행기관

American Chemical Society

라이선스

cc-by-nc

ISSN

0021-8561

ISSN

1520-5118

권

호