BioChem - DOS

Quercetin production from rutin by a thermostable β-rutinosidase from Pyrococcus furiosus

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

Nam, Hyun-Koo; Hong, Seung-Hye; Shin, Kyung-Chul; Oh, Deok-Kun

<P>Pyrococcus furiosus β-glucosidase converted rutin to quercetin and rutinose disaccharide with a ratio of 1:1, with no glucose, L-rhamnose, and isoquercitrin, indicating that the enzyme is a β-rutinosidase. The specific activity for flavonoid glycosides followed the order of isoquercitrin > quercitrin > rutin. The conversion of rutin to quercetin was optimal at pH 5.0 and 95C in the presence of 0.5% dimethyl sulfoxide with a half-life of 101 h, a k(cat) of 1.6 min(-1), and a K(m) of 0.3 mM. Under the improved conditions, the enzyme produced 6.5 mM quercetin from 10 mM rutin after 150 min, with a molar yield of 65% and a productivity of 2.6 mM/h. This productivity is the highest reported thus far among enzymatic transformations.</P>

2012

Springer-Verlag

0141-5492

1573-6776

34

3

pp.483-489

10.1007/s10529-011-0786-2

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58442748