초록
<P><B>Abstract</B></P> <P>The hydrogen production utilizing photosynthetic and anaerobic bacteria in two-stage approach has many drawbacks, such as shortage of raw materials and complexity of operations. Accordingly, we aimed to develop a simple one-stage H<SUB>2</SUB> production protocol using the depolymerization of maize straw cellulose as a cheap carbon source. <I>R. sphaeroides</I> HY01 and its mutant (<I>Hup</I> <SUP> <I>−</I> </SUP>) were studied regarding their H<SUB>2</SUB> production under different culture conditions. Further study using two model sugars, their combination, and straw hydrolysate as carbon sources was conducted to determine the effects of substrate on H<SUB>2</SUB> production. When using the straw hydrolysate as carbon source, the pH remained in a range of 7.1–7.6, whereas it dropped to 5.4–7.4 when using the model sugars, and the former biomass value was greater. The H<SUB>2</SUB> production performance of the mutant was significantly better than that of HY01. One-step photo-fermentative H<SUB>2</SUB> production was superior when using straw hydrolysate as opposed to the simple model sugars, and its yield was up to 4.62 mol H<SUB>2</SUB>·mol<SUP>−1</SUP> reducing sugar.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We explored one-stage photo fermentation of the conversion from maize straw to bioH2. </LI> <LI> The hydrogen performance of the mutant (<I>HupSL</I> <SUP> <I>−</I> </SUP>) of <I>R. sphaeroides</I> were enhanced. </LI> <LI> The pH had a self-stabilization effect when using the straw hydrolysate as substrate. </LI> <LI> H<SUB>2</SUB> production was superior when using straw hydrolysate as opposed to model sugars. </LI> </UL> </P>