초록
<P><B>Abstract</B></P><P>In the present study, the photo-hydrogen production performances by <I>Rhodopseudomonas palustris</I> CQK 01 growing from the inoculated cells with ultra-sonication pretreatment (<I>R. palustris</I> CQK 01-USP) were experimentally investigated in batch culture and compared with that without pretreatment (<I>R. palustris</I> CQK 01-NP). It was found that the ultra-sonication pretreatment modified membrane morphology and broke up part of the cells, resulting in improvement of membrane permeability and bacterial activities and hence, helping the improvement of hydrogen production. The hydrogen production rate, hydrogen yield and energy conversion efficiency with <I>R. palustris</I> CQK 01-USP were increased to be nearly 2 times higher than that with <I>R. palustris</I> CQK 01-NP. The parametric study showed that under the conditions of initial glucose concentration 50mmol/l, inoculum size 12%, illumination wavelength 590nm, the photobioreactor with <I>R. palustris</I> CQK 01-USP obtained the optimal hydrogen production rate 0.54mmol/l/h, hydrogen yield 1.2mol-H<SUB>2</SUB>/mol-glucose and energy conversion efficiency 9.03%.</P>