초록
<P><B>Abstract</B></P> <P> <I>Bacillus aryabhattai</I> BA03, a strain isolated in our laboratory, has interesting properties related to the production of natural aromas and flavors. Specifically, we have found that it was able to produce vanillin from ferulic acid (FA). Furthermore, this strain produces high amounts of 4-vinylguaiacol in only 14h, this being the only intermediate metabolite observed in the process. FA is an inexpensive feedstock for the production of natural value-added compounds when extracted from lignocellulosic wastes. In this study, we optimized the operational conditions (temperature, pH and agitation), medium composition and bioconversion technology (batch or fed-batch) to produce vanillin. In a fed-batch process conducted with just one additional supplementation after 24h, the maximal concentration of vanillin (147.1±0.9mg/L) was observed after 216h (<I>Q<SUB>V</SUB> </I> =0.681mg/Lh; <I>Y<SUB>V/fFA</SUB> </I> =0.082mg/mg) after degrading 90.3% FA. In view of our data, we postulate that <I>Bacillus aryabhattai</I> BA03 carries out a decarboxylation of ferulic acid as a metabolic pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Bacillus aryabhattai</I> BA03 is a strain able to grow under a wide range of conditions. </LI> <LI> Operational conditions to vanillin production from FA were 37°C, 150rpm and pH 8.5. </LI> <LI> The most suitable medium was that formulated with only 10g/L yeast extract. </LI> <LI> Fed-batch process allowed obtain the better results to vanillin production. </LI> <LI> 4-vynilguaiacol was the only intermediate observed in the process. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>