초록
<P><B>Abstract</B></P> <P>This study has investigated ultrasound-assisted xylitol production through fermentation of dilute acid (pentose-rich) hydrolysate of sugarcane bagasse using free cells of <I>Candida tropicalis</I>. Sonication of fermentation mixture at optimum conditions was carried out in ultrasound bath (37 kHz and 10% duty cycle). Time profiles of substrate and product in control (mechanical shaking) and test (mechanical shaking + sonication) fermentations were fitted to kinetic model using Genetic Algorithm (GA) optimization. Max. xylitol yield of 0.56 g/g and 0.61 g/g of xylose was achieved in control and test fermentations, respectively. The biomass yield also increased marginally (∼17%) with sonication. However, kinetics of fermentation increased drastically (2.5×) with sonication with 2× rise in xylose uptake and utilization by the cells. With comparative analysis of kinetic parameters in control and test experiments, this result was attributed to enhanced permeability of cell membrane that allowed faster diffusion of nutrients, substrates and products across cell membrane, higher enzyme-substrate affinity, dilution of toxic components and reduced inhibition of intracellular enzymes by substrate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ultrasound-assisted xylitol fermentation using free cells of <I>Candida tropicalis</I>. </LI> <LI> Time profiles of substrate and product fitted to kinetic model using Genetic Algorithm. </LI> <LI> Max. xylitol yield of 0.56 g/g and 0.61 g/g of xylose in control and test fermentations. </LI> <LI> Kinetics of fermentation increased 2.5× with sonication at 37 kHz (10% duty cycle). </LI> <LI> Sonication causes enhanced cell permeability and higher enzyme-substrate affinity. </LI> </UL> </P>