BioChem - DOS

Mesophilic continuous fermentative hydrogen production from acid pretreated de-oiled jatropha waste hydrolysate using immobilized microorganisms

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Kumar, Gopalakrishnan; Sivagurunathan, Periyasamy; Sen, Biswarup; Kim, Sang-Hyoun; Lin, Chiu-Yue

<P><B>Abstract</B></P> <P>Mesophilic hydrogen production from acid pretreated hydrolysate (biomass concentration of 100g/L and 2% hydrochloric acid) of de-oiled jatropha waste was carried out in continuous system using immobilized microorganisms at various hydraulic retention times (HRTs) ranging from 48 to 12h. The experimental results of the reusability of immobilized microorganisms showed their stability up to 10 cycles with an average cumulative hydrogen production of 770mL/L. The peak hydrogen production rate and hydrogen yield were 0.9L/L*d and 86mL/g<SUB>reducing sugars added</SUB>, respectively at 16h HRT, with butyrate as the predominant volatile fatty acid. The microbial community analysis revealed that majority of the PCR-DGGE bands were assigned to genus <I>Clostridium</I> and were perhaps the key drivers of the higher hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 2% HCl-acid pretreated DJW hydrolysate CSTR-H<SUB>2</SUB> fermentation was demonstrated. </LI> <LI> Continuous operation was success until 16h HRT. </LI> <LI> Peak hydrogen production rate (HPR) was attained as 0.9L/L*d. </LI> <LI> PCR-DGGE bands represent dominant population as <I>Clostridium</I> spp. </LI> </UL> </P>

2017

Elsevier

0960-8524

240

pp.137-143

10.1016/j.biortech.2017.03.059

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78030992

Acid pretreated hydrolysate; Deoiled jatropha waste; Immobilization; Soluble metabolic products; PCR-DGGE