BioChem - DOS

On-line monitoring of Aspergillus niger GH1 growth in a bioprocess for the production of ellagic acid and ellagitannase by solid-state fermentation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Aguilar-Zá rate, Pedro; Wong-Paz, Jorge E.; Rodrí guez-Duran, Luis V.; Buenrostro-Figueroa, Juan; Michel, Mariela; Saucedo-Castañ eda, Gerardo; Favela-Torres, Ernesto; Ascacio-Valdé s, Juan A.; Contreras-Esquivel, Juan C.; Aguilar, Cristó bal N.

<P><B>Abstract</B></P> <P>The present work describes the monitoring of CO<SUB>2</SUB> production by <I>Aspergillus niger</I> GH1 in a bioprocess for the production of ellagitannase (EAH) and ellagic acid by solid state fermentation. Pomegranate ellagitannins, mainly punicalagin, were used as carbon source and EAH inducer. A second condition, using ellagitannins and maltose as growth promoting carbon source, was tested. The ellagic acid production was quantified and the EAH activity was assayed. The accumulated metabolites were identified by HPLC-ESI-MS/MS. Higher CO<SUB>2</SUB> production (7.79mg/grams of dry material) was reached in media supplemented with maltose. Short-time lag phase (7.79h) and exponential phase (10.42h) were obtained using only ellagitannins, despite its lower CO<SUB>2</SUB> production (3.79mg/grams of dry material). Without the use of maltose lower ellagic acid (11.85mg/L/h) and EAH (21.80U/L/h) productivities were reached. The use of maltose enhances the productivity of EA (33.18mg/L/h) and EAH (33.70U/L/h). Besides of punicalin and ellagic acid, two unknown compounds with mass weight of 702 and 290g/mol (ions 701 and 289 <I>m/z</I> in negative mode, respectively) were identified and characterized by HPLC-ESI-MS/MS analysis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CO<SUB>2</SUB> evolution was monitored in a bioprocess to produce ellagitannase and ellagic acid in SSF. </LI> <LI> The use of maltose enhances the productivity of the process. </LI> <LI> Two unknown compounds (M.W. 702 and 290g/mol) were identified and characterized by HPLC-MS/MS analysis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

0960-8524

247

pp.412-418

10.1016/j.biortech.2017.09.115

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79612514

CO2; Fungal growth; Respirometry; Pomegranate ellagitannins