초록
<P><B>Abstract</B></P> <P>The aim of present work was to effectively remediate grease waste by <I>Penicillium chrysogenum</I>. For efficient degradation, grease waste was pre-treated using various lipases, among them lipolase was the best. The pretreated grease was used as a substrate by <I>P. chrysogenum</I> resulting into the production of fatty acids. Process was optimized by response surface methodology (RSM) using four variables <I>viz</I>; FeCl<SUB>2</SUB> (mM), spore concentration (spores/ml), time period (days) and amount of grease (g). The optimized conditions <I>viz;</I> FeCl<SUB>2</SUB> 1.25mM, culture amount 5×10<SUP>11</SUP> spores/ml and time period 16days led to the production of 6.6mg/g fatty acid from 10.0g of pre-treated grease mixed with 5.0g wheat bran in 10.0ml czapek-dox medium under solid state fermentation. The fermented media was extracted with hexane and subjected to GCMS analysis, which showed the presence of higher amount of palmitic acid. It was purified by crystallization method and 2.8g of palmitic acid was recovered from 1.0kg grease waste in tray fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pre-treatment is needed to bio-remediate grease waste. </LI> <LI> <I>Penicillium chrysogenum</I> can utilize pre-treated grease as a sole carbon source. </LI> <LI> Fatty acid was purified by crystallization methods and analysed by GC–MS. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>