Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
기타
논문
Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation
학술지
Catalysis communications
저자명
Liu, M.q.; Dai, X.j.; Guan, R.f.; Xu, X.
초록
Aspergillus niger xylanase A (XylA) was immobilized onto Fe<SUB>3</SUB>O<SUB>4</SUB>-coated chitosan magnetic nanoparticles prepared by the layer-by-layer self-assembly approach. The Fe<SUB>3</SUB>O<SUB>4</SUB>-coated chitosan magnetic nanoparticles showed a high binding capacity of 162.2mg@?g<SUP>-1</SUP>-particles and a recovery activity of 56.5% for XylA. The immobilized XylA showed improved thermostability and storage stability compared with free XylA. The immobilized XylA retained 87.5% activity after seven successive reactions by magnetic separation. Xylotriose and xylohexaose were the main products released from birchwood xylan and wheat bran insoluble xylan by immobilized XylA, respectively.