BioChem - DOS

Effect of reduced sulfur compounds on the fermentation of phosphoric acid pretreated sugarcane bagasse by ethanologenic Escherichia coli

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Nieves, I.U.; Geddes, C.C.; Miller, E.N.; Mullinnix, M.T.; Hoffman, R.W.; Fu, Z.; Tong, Z.; Ingram, L.O.

The addition of reduced sulfur compounds (thiosulfate, cysteine, sodium hydrosulfite, and sodium metabisulfite) increased growth and fermentation of dilute acid hydrolysate of sugarcane bagasse by ethanologenic Escherichia coli (strains LY180, EMFR9, and MM160). With sodium metabisulfite (0.5mM), toxicity was sufficiently reduced that slurries of pretreated biomass (10% dry weight including fiber and solubles) could be fermented by E. coli strain MM160 without solid-liquid separation or cleanup of sugars. A 6-h liquefaction step was added to improve mixing. Sodium metabisulfite also caused spectral changes at wavelengths corresponding to furfural and soluble products from lignin. Glucose and cellobiose were rapidly metabolized. Xylose utilization was improved by sodium metabisulfite but remained incomplete after 144h. The overall ethanol yield for this liquefaction plus simultaneous saccharification and co-fermentation process was 0.20g ethanol/g bagasse dry weight, 250 L/tonne (61gal/US ton).

2011

Elsevier Applied Science

0960-8524

102

8

pp.5145-5152

10.1016/j.biortech.2011.02.008

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55729704

Sodium metabisulfite; Lignocellulose; SSF; L+SScF; Ethanol