초록
Co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production. This study evaluated potential of apple pomace hydrolysate (APH) co-fermented with crude glycerol (CG) for increased H<SUB>2</SUB> production and decreased by-products formation. The central composite design (CCD) along with response surface methodology (RSM) was used as tool for optimization and 15g/L of CG, 5g/L of APH and 15% (v/v) inoculum were found to be optimum to produce as high as 26.07+/-1.57mmol H<SUB>2</SUB>/L of medium. The p-value of 0.0017 indicated that APH at lower concentration had a significant effect on H<SUB>2</SUB> production. By using CG as sole carbon source, reductive pathway of glycerol metabolism was favored with 19.46mmol H<SUB>2</SUB>/L. However, with APH, oxidative pathway was favored with higher H<SUB>2</SUB> production (26.07+/-1.57mmol/L) and decrease in reduced by-products (1,3-propanediol and ethanol) formation. APH inclusion enhanced H<SUB>2</SUB> production, and decreased substrate inhibition.