Feasibility of reed for biobutanol production hydrolyzed by crude cellulase
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
치료제
식품첨가제
논문
Feasibility of reed for biobutanol production hydrolyzed by crude cellulase
학술지
Biomass & bioenergy
저자명
Zhu, Youshuang; Xin, Fengxue; Chang, Yunkang; Zhao, Ying; Weichong, Wong
초록
<P><B>Abstract</B></P> <P>The aim of this study was to efficiently utilize reed for both cellulase and biobutanol production. The unprocessed cellulase blend produced under solid-state fermentation using reed as the substrate showed a similar reducing sugar yield using Whatman filter paper to the commercial enzyme blend (38.61%). Organosolv pretreatment method could efficiently reduce hemicellulose (29.3%–14.6%) and lignin (17.2%–14.1%) content and increase cellulose content (42.5%–62.3%) from reed. Enzymatic hydrolysis of organosolv-pretreated reed using the crude cellulase with enzyme loading of 25 FPU/g reed, 20% solid content at 50 °C and pH 5.5 resulted in a reed hydrolysate containing 40.01 g/L glucose and 3.55 g/L xylose after 72 h. Fermentation of the hydrolysate medium by <I>Clostridium acetobutylicum</I> produced 9.07 and 14.24 g/L of biobutanol and ABE with yield of 0.21 g/g and 0.33 g/g, respectively. This study proved that crude cellulase complex produced under solid state fermentation and organsolv pretreatment can efficiently provide reed hydrolysate that can be converted to biobutanol without any commercial cellulase usage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 9.07 g L<SUP>−1</SUP> butanol with yield of 0.21 g g<SUP>−1</SUP> was produced from reed hydrolysate. </LI> <LI> 23.42 FPU g<SUP>−1</SUP> of FPase could be produced under solid state fermentation using reed as the substrate. </LI> <LI> Organosolv pretreatment could efficiently remove hemicellulose and improve cellulose content of reed. </LI> </UL> </P>