초록
<P><B>Abstract</B></P> <P>Solid state fermentation (SSF) is used to produce industrial enzymes. The objective of this study was to use a co-culture of <I>Aspergillus niger</I> GS1 and <I>Trichoderma reesei</I>, grown on a mixture of Bermuda grass and corn cob to obtain fermented forage (FF) rich in hydrolytic enzymes, as a value added ingredient for animal feed. FPase, amylase and xylanase productivities (dry matter, DM) were 8.8, 181.4, and 42.1Ug<SUP>−1</SUP> h<SUP>−1</SUP>, respectively (1U=reducing sugars released min<SUP>−1</SUP>), after 12–16h of SSF with C/N=60. Cellulose, hemicellulose and lignin decreased 1.6-, 2.7- and 1.9-fold (DM), respectively. <I>In vitro</I> ruminal and true digestibility of DM was improved 2.4- and 1.4-fold. Ruminal digestion of FF reduced 1.32-fold the acetate:propionate ratio, which may reduce the environmental impact of ruminants feeding. On-site hydrolytic enzymes productivity using SSF without enzymes extraction could be of economic potential for digestibility improvement in animal feed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> On-site enzymes were produced from fungal fermentation of Bermuda grass and corn cob. </LI> <LI> Fermented forage showed high cellulolytic, amylolytic and xylanolytic productivities. </LI> <LI> Fermented forage showed potential in ruminal digestibility improvement of animal feed. </LI> <LI> On-site enzymes production could represent economical availability of animal feed. </LI> </UL> </P>