초록
<P><B>Abstract</B></P> <P>This study investigates the effects of ethanol prefermentation (EP) on methane fermentation. Yeast was added to the substrate for EP in the sequencing batch methane fermentation of food waste. An Illumina MiSeq high-throughput sequencing system was used to analyze changes in the microbial community. Methane production in the EP group (254mL/g VS) was higher than in the control group (35mL/g VS) because EP not only increased the buffering capacity of the system, but also increased hydrolytic acidification. More carbon source was converted to ethanol in the EP group than in the control group, and neutral ethanol could be converted continuously to acetic acid, which promoted the growth of <I>Methanobacterium</I> and <I>Methanosarcina</I>. As a result, the relative abundance of methane-producing bacteria was significantly higher than that of the control group. Kinetic modeling indicated that the EP group had a higher hydrolysis efficiency and shorter lag phase.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ethanol prefermentation (EP) can increase buffering capacity. </LI> <LI> EP group achieved higher pH (6.8–7.2) than control group (6.1–6.75). </LI> <LI> Lag phase in EP group was 1.3d, less than the control group for 3.3d. </LI> <LI> Average propionic acid concentration in EP group was 0.43g/L, decreased by 54.7%. </LI> <LI> Methanobacterium became the dominant species with relative abundance 57.03%. </LI> </UL> </P>