Chi, Z.; Rover, M.; Jun, E.; Deaton, M.; Johnston, P.; Brown, R.C.; Wen, Z.; Jarboe, L.R.
초록
The application of pyrolytic sugars for biofuel production through fermentation is challenged by inhibitory contaminant compounds. Inhibition is so severe that only 0.25% sugar syrup can be used. In this study, overliming was tested as a simple detoxification method, using the Escherichia coli KO11+ lgk to directly convert levoglucosan into ethanol. After treatment with at least 14.8g/L of Ca(OH)<SUB>2</SUB>, fermentation with 2% (w/v) pyrolytic sugar syrup was observed with no inhibition of ethanol production. Further investigation of treatment time and temperature showed that 8-16h of treatment at 20<SUP>o</SUP>C, and 1-4h of treatment at 60<SUP>o</SUP>C are necessary to obtain consistent ethanol production. The samples treated with 18.5g/L Ca(OH)<SUB>2</SUB> at 60<SUP>o</SUP>C for 4h showed no inhibition at 2.5%. Multiple contaminants removed by the overliming treatment were identified. This study demonstrates that overliming is a promising method for detoxification of pyrolytic sugars for fermentation.