초록
<P><B>Highlights</B></P><P>► <I>Trichoderma reesei</I> RT-P1 is a native enzyme powder. ► The enzyme powder is cheap to produce and easy to use. ► Lignocellulosic materials are hydrolyzed by <I>T. reesei</I> RT-P1 without pre-treatment. ► Ethanol concentration is 50g/L at 8days fermentation.</P> <P><B>Abstract</B></P><P>Use of <I>Trichoderma reesei</I> RT-P1 crude enzyme powder and of this powder with 10%v/v <I>Saccharomyces cerevisiae</I> for ethanol fermentation of sweet sorghum fresh stalks were investigated. The optimal conditions were determined by orthogonal experiment method. With <I>T. reesei</I> crude enzyme powder, the optimal condition for the Keller cultivar was at 25g with 4g enzyme loading and for the Cowley cultivar at 30g with 5g enzyme loading, both with 8days fermentation at pH 5 and 30°C. At the optimal conditions above, ethanol concentration, productivity and yield of the Cowley cultivar (35.00g/L, 0.18g/Lh and 0.38g ethanol/g substrate, respectively) were higher than those of the Keller cultivar (20.46g/L, 0.11g/Lh and 0.28gethanol/gsubstrate). The addition of 10%v/v <I>S. cerevisiae</I> to fermentation at the optimal conditions showed no significant variations in ethanol concentration, productivity and yield for both cultivars.</P>