BioChem - DOS

Production of polyunsaturated fatty acids by Mortierella alpina using submerse and solid state fermentation

Chemie-Ingenieur-Technik

Stressler, Timo; Eisele, Thomas; Rost, Johanna; Haunschild, Eva‐ Maria; Kuhn, Andreas; Fischer, Lutz

<P><B>Abstract</B></P><P>A new isolate of <I>Mortierella alpina</I>, &amp;&amp;num;62;？98&thinsp;% identical with <I>M. alpina</I> ATCC 16266, was cultivated in a defined glucose&#8208;based medium with three organic nitrogen sources (glycine, urea and Na&#8208;<I>L</I>&#8208;glutamate) at three different concentrations in shaking flasks at 20&thinsp;&deg;C. The results were compared to the cultivation in complex medium with yeast extract as nitrogen source. In the defined media, high yields of polyunsaturated fatty acids (PUFAs) and arachidonic acid (ARA), respectively, were obtained with Na&#8208;<I>L</I>&#8208;glutamate. However, the absolute highest yields of PUFA and ARA were measured with the yeast extract medium. An optimized yeast extract complex medium was used for a submerse bioreactor cultivation in a 45&#8208;L scale. Furthermore, <I>M. alpina</I> was cultivated in a solid state fermenter, using an oat bran water mixture as substrate.</P>

2013

WILEY&#x2010;VCH Verlag

0009-286x

1522-2640

85

3

pp.318-322

10.1002/cite.201200094

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65590324

Arachidonic acid; Complex medium; Defined medium; Mortierella alpina; Polyunsaturated fatty acids; Solid state fermentation; Submerse cultivation