초록
The indigenous bacteria, cow dung and sewage sludge were used as seed microflora sources to ferment sweet potato into hydrogen and ethanol under batch cultivation at a temperature of 37<SUP>o</SUP>C. Hydrogen and ethanol production was evaluated based on their yields (HY and EY) and production rate (HPR and EPR) at various sweet potato concentrations (30-240g/L), sweet potato particle diameters (<1.19mm to >6.38mm) and initial cultivation pH conditions (4.0-9.0). The experimental results indicate an interesting finding that sweet potato fermentation without adding external seed (i.e., the fermenter contained only the indigenous bacteria which were identified to have seven species) could produce hydrogen and ethanol (EtOH). The indigenous bacterial community analysis showed that Klebsiella oxytoca, Clostridium fimetarium, Grimontella senegalensis, and Enterobacter asburiae (or Escherichia coli) were present at peak ethanol production and two more species Ruminococcus schinkii and Lactovum miscens were present at peak hydrogen production. The externally seeding strategy could enhance the hydrogen production from sweet potato with peak cumulative H<SUB>2</SUB> production potential (HP) of 120mmolH<SUB>2</SUB>/L-reactor using sewage sludge. However, the cow dung seed showed more ethanol production than hydrogen with peak concentration of 12,146mgCOD/L. Increasing the sweet potato concentration could enhance the HPR and HP values. Maximum HY of 1.24molH<SUB>2</SUB>/molhexose was obtained at a substrate concentration of 150g/L with an initial cultivation pH of 6.7 and particle diameter <1.19mm using sewage sludge. The HPR<SUB>max</SUB>, HY and HP values were affected slightly by the variations in sweet potato particle size. The peak total energy production yield of 1625J/g sweet potato was obtained with sweet potato concentration of 150g/L at pH 8.5 using sewage sludge.